Human Tri-iodothyronine(T3) ELISA Kit
Product Description
This kit uses enzyme-linked immune sorbent assay (ELISA) based on biotin double antibody sandwich technology to assay Human Tri-iodothyronine(T3). Add Tri-iodothyronine(T3) to wells that are pre-coated with Tri-iodothyronine(T3) monoclonal antibody and then incubate. After incubation, add anti Tri-iodothyronine(T3) antibodies labeled with biotin to unite with Streptavidin-HRP, which forms the immune complex. Remove unbound enzymes after incubation and washing, then add substrate A and B. The solution will turn blue and change to yellow with the effect of acid. The shades of solution and the concentration of Human Tri-iodothyronine(T3) are positively correlated.
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