| Model: | - |
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| Brand: | - |
| Origin: | Made In United States |
| Category: | Chemicals / Pharmaceutical Chemicals |
| Label: | 3-CAT elisa kit , Human elisa kit , elisa kit |
| Price: |
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| Min. Order: | - |
| Live Chat: | Last Online:16 Nov, 2018 |
Purpose
This kit allows for the determination of 3-CAT concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human 3-CAT level in the sample，use Purified Human 3-CAT antibody to coat microtiter plate wells, make solid-phase antibody, then add 3-CAT to wells, Combined 3-CAT antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-CAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
