Model: | - |
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Brand: | - |
Origin: | Made In United States |
Category: | Chemicals / Pharmaceutical Chemicals |
Label: | Bovine , Prostaglandin F2a , elisa kit |
Price: |
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Min. Order: | - |
Live Chat: | Last Online:16 Nov, 2018 |
Purpose
This kit allows for the determination of PGF2a concentrations in Bovine serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Bovine PGF2a level in the sample,use Purified Bovine PGF2a antibody to coat microtiter plate wells, make solid-phase antibody, then add I PGF2a to wells, Combined PGF2a antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGF2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.