Home > Products > Chemicals > Pharmaceutical Chemicals

The AG 96 Well DNA Binding Column is designed for high-throughput purification of DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

Purify 96 wells of pure genomic DNA in 45 minutes!

Sample Volume: up to 200 µl of whole blood, plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured cells per well

Yield: up to 6 μg of genomic DNA from 200 µl of whole blood per well 

Format: 96 Well Binding Plates

Elution Volume: 200 ~ 400 µl

Centrifuge or vacuum manifold protocol

Storage: dry at room temperature (15-25ºC)

HiPure FFPE DNA Column  #D51011

The HiPureFFPE DNA Column provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using Proteinase K, heat, and then purify the DNA  with the FFPE DNA Column. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions with protocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.