| Model: | D40011 |
|---|---|
| Brand: | Angen Biotech |
| Origin: | Made In China |
| Category: | Chemicals / Pharmaceutical Chemicals |
| Label: | - |
| Price: |
US $0.34
/ pc
|
| Min. Order: | 1000 pc |
| Live Chat: | Last Online:12 Jul, 2019 |
HiPure DNA Micro Column provides for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.
|
DNA Recovery |
Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. |
|
Equipment |
microcentrifuge, vortex |
|
DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
|
Sample Sources |
Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed. |
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DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
|
Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
|
Workflow Overview |
Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
