| Model: | D50081 |
|---|---|
| Brand: | Angen Biotech |
| Origin: | Made In China |
| Category: | Chemicals / Pharmaceutical Chemicals |
| Label: | - |
| Price: |
US $180
/ pc
|
| Min. Order: | 1000 pc |
| Live Chat: | Last Online:12 Jul, 2019 |
AG-CFDNA Column Set provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A).
The AG-CFDNA Column Set contains with
20ml Funnel Column
CFDNA Mini Column
Collection Tube III
50ml Centrifuge Tube
|
DNA Recovery |
Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
|
Equipment |
Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
|
Sample Sources |
Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
|
DNA Purity |
High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
|
Storage |
Eluted DNA should be stored at ≤ -20ºC. |
|
Recovery Volume |
≥ 35 μl of DNA Elution Buffer or DNase free water. |
|
Processing Volume |
Plasma – Single centrifugation: up to 3 ml
|
