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Glucoamylase is inverting exo-acting starch hydrolases releasing beta-glucose from the non-reducing ends of starch and related substrates. The majority of glucoamylases are multidomain enzymes consisting of a catalytic domain connected to a starch-binding domain by an O-glycosylated linker region. Three-dimensional structures have been determined of free and inhibitor complexed glucoamylases from Aspergillus awamori var. X100, Aspergillus niger, and Saccharomycopsis fibuligera. The catalytic domain folds as a twisted (alpha/alpha)(6)-barrel with a central funnel-shaped active site, while the starch-binding domain folds as an antiparallel beta-barrel and has two binding sites for starch or beta-cyclodextrin.

Boli GA is widely applied to the production of alcohol, fuel ethanol, glucose, antibiotics and brewing industries. It is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca.

This product is available both in solid (powder) and liquid forms.

Product Characteristics

Declared Enzyme:	Glucoamylase
Systematic Name:	EC 3.2.1.3,1,4-alpha-D-glucan glucohydrolase
Physical Form:	Liquid                                           Solid powder
Activity:	100,000～150,000 U/ml          100,000～200,000 U/g
Appearance:	Light to dark brown                    Light yellow powder
PH or Moisture:	3.0 to 5.0                                     ＜8%
Specific gravity:	1.10 to 1.25 g/ml

Effect of PH

For maximum activity, the optimal pH for Boli GA is in the range of 3.5 to 4.5.

Effect of Temperature

The optimal temperature range for Boli GA is 58～65°C(137～149°F).Temperatures above 65°C (149°F) can result in loss of activity.

Benefits

• Faster fermentation
• More output of ethanol
• Highly efficient in starch/dextrin degradation
• Greatly improved performance in pre-saccharification processes
• Less starch residues

Dosage Guidelines

Boli GA is generally added at a level of 0.062～0.077% w/w in ethanol processing (starch, dry solids basis, provided that the enzyme activity is 150,000 U/ml). The actual dosage depends on the raw materials, temperature, reaction time, and pH of the individual plant saccharification/fermentation process, and its desired result.