Rat Tumor necrosis factor α(TNF-α) ELISA Kit
Product Description
Purpose
This kit allows for the determination of TNF-α concentrations in Rat serum, tissue and other biological fluids.
Principle of the assay
The kit assay Rat TNF-α level in the sample，use Purified Rat TNF-α to coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined antibody which With HRP labeled goat anti-Rat become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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