Fish Glutathione S-transferases(GSTs)ELISA Kit
Product Description
【Intended Use】
This kit is used to assay the Glutathione S-transferases(GSTs)in the sample of Fish’s serum, blood plasma and other related biological liquid.
【Test principle】
This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Fish Glutathione S-transferases (GSTs). Add Glutathione S-transferases(GSTs)to the wells, which are pre-coated with Glutathione S-transferases(GSTs)monoclonal antibody and then incubate. After that, add anti GSTs antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Fish Glutathione S-transferases (GSTs) are positively correlated.
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