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Purpose

This kit allows for the determination of ANGⅡR-1 concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human ANGⅡ R-1 level in the sample，use Purified Human ANGⅡR-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add ANGⅡR-1 to wells, Combined ANG Ⅱ R-1 antibody which With HRP labeled goat anti-Human become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANG Ⅱ R-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.