| Model: | E0079h |
|---|---|
| Brand: | EIAab |
| Origin: | Made In China |
| Category: | Chemicals / Chemical Reagent |
| Label: | research kits , Assay kits , IL-6/Interleukin-6 |
| Price: |
US $276
/ box
|
| Min. Order: | 1 box |
| Live Chat: |  | Last Online:18 May, 2022 |
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of canine interleukin 6, IL-6 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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