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Biological buffer is usually a very common auxiliary reagent used in biochemical detection and biological experiments. For example: Bicine, Bicine buffer has the characteristics of maintaining the pH value of the system stable, ensuring the reaction, promoting the activity of enzymes, and stabilizing the structure of protein and nucleic acid. One of the key functions is to buffer the change of the pH of the system. At this time, the pH value of the Bicine buffer becomes particularly important, but sometimes the pH value is changed due to the influence of some factors. At this time, special attention needs to be paid to it. What determines its pH value?
1. PH value is determined by pKa
The pH value of Bicine buffer is determined by the pKa of the conjugate acid-base pair that constitutes the buffer and the buffer ratio, but the pKa plays a decisive role, and the buffer ratio has less influence. Therefore, after formulating a buffer solvent, if the buffer agent to be selected is not determined, it is first necessary to find the buffer agent corresponding to the buffer range according to the required pH value. The closer the pKa of the buffer agent is to the required pH value, the better. In addition, it is necessary to pay attention to whether the buffer has an adverse reaction to the system reaction. If the pH value of a variety of buffers meets the requirements, a buffer with good solubility, non-toxic and harmless, strong stability and easy storage can be selected.
2. The pH value is determined by adjusting the buffer ratio
If the buffer is determined, the change of its pH value is sometimes affected by the adjustment of the buffer ratio. For example, if the pH value of Bicine increases, the proportion of sodium hydroxide will increase, and if the pH value decreases, the proportion of Bicine will increase.
3. PH value is determined by buffer concentration, ionic strength and osmotic pressure
The higher the concentration of the buffer solution, the better the buffering effect and the stronger the buffering capacity. However, it should be noted that the higher the concentration, the better. When determining the preparation of the buffer solution, the pH value will also be affected by the total concentration and the need Consider the effects of ionic strength and osmotic pressure on the reaction system.
The above three points are the key factors in determining the pH range of Bicine buffer solution, and more attention should be paid during use. Desheng Biochemical is a professional manufacturer of biological buffers, such as the more commonly used Tris, Bicine, MOPS, CAPS, HEPES, etc., all of which are produced, which can meet the needs of most biological experiments, and the company has a large number of stock, according to customer needs on the same day Delivery, quality is also well received by customers, exported to overseas. If you have requirements for biological buffers, please click on the website for more information!