| Model: | CK-E10005 |
|---|---|
| Brand: | eastibiopharm |
| Origin: | Made In China |
| Category: | Chemicals / Biochemical |
| Label: | elisa kit , elisa test kit , diagnostic test kit |
| Price: |
US $400
/ pc
|
| Min. Order: | 1 pc |
| Live Chat: | Last Online:08 Sep, 2015 |
Intended Use:
This kit is used to assay the sample of Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.
Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Chicken adrenomedullin,ADM elisa kits samples. Add Chicken adrenomedullin,ADM elisa kits monoclonal antibody Enzyme well which is pre-coated with Chicken adrenomedullin,ADM elisa kits monoclonal antibody, incubation; then, add Chicken adrenomedullin,ADM elisa kits antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Substance Chicken adrenomedullin,ADM elisa kits sample were positively correlated.
Materials supplied in the Test Kit
|
1 |
Standard |
0.5ml |
7 |
Chromogen Solution A |
6ml |
|
2 |
Standard diluent |
3ml |
8 |
Chromogen Solution B |
6ml |
|
3 |
Microelisa Stripplate |
12w×8s |
9 |
Stop Solution |
6ml |
|
4 |
Str- HRP-Conjugate Reagent |
6ml |
10 |
Instruction |
1 |
|
5 |
30×wash solution |
20ml |
11 |
Closure plate membrane |
2 |
|
6 |
Biotin-Ab |
1ml |
12 |
Sealed bags |
1 |
Materials required but not supplied
1. 37 °C incubator
2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips
4. Distilled water
5. Disposable tubes for sample dilution
6. Absorbent paper
Summary Procedure
Preparing reagents, samples and standards
Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at 37 °C
Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at 37 °C
Add stop solution
Measure the OD value within 10min
Package size:96T per box.
Validity&Storage:Six months(2-8°C)or Twelvemonths(-20°C)
[see label on the outer box for the specific date].
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