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Intended Use
This kit is used to assay the Immunoglobulin G4(IGG4) in the sample of human’s serum, blood plasma, and other related tissue Liquid.

Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Immunoglobulin G4(IGG4) in samples. Add Immunoglobulin G4(IGG4) to monoclonal antibody Enzyme well which is pre-coated with Human Immunoglobulin G4(IGG4)monoclonal antibody, incubation; then, add Immunoglobulin G4(IGG4)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Immunoglobulin G4(IGG4) of sample were positively correlated.