Nitrofurazone(SEM)ELISA Test Kit
Product Description
REAGEN™ Nitrofurazone (SEM) ELISA Test Kitis a competitive enzyme immunoassay for the quantitative analysis of Nitrofurazone in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The SEM antibody has been coated in the plate wells. During the analysis, sample is added along with the SEM-horseradish peroxidase (SEM-HRP) conjugate. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the SEM-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample.
Kit Contents, Storageand Shelf Life
REAGEN™ Nitrofurazone (SEM) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents
|
Amount
|
Storage
|
|
SEM Antibody-Coated Plate
|
96-well plate (8 wells x 12 strips)
|
2-8°C
|
|
SEM Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.2ng/mL (orange cap tube)
0.8 ng/mL (pink cap tube)
3.2 ng/mL (purple cap tube)
9.6 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
|
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
|
2-8°C
|
|
SEM- HRP Conjugate
|
6 mL
|
2-8°C *
|
|
10X Sample Extraction Buffer **
|
25 mL
|
2-8°C
|
|
20X Wash Solution **
|
28 mL
|
2-8°C
|
|
Stop Buffer **
|
20 mL
|
2-8°C
|
|
TMB Substrate **
|
12 mL
|
2-8°C
|
|
50 mM 2-Nitrobenzaldehyde **
|
1.8 mL
|
2-8°C
|
* If you are not planning to use the kit for over 1 month, store SEM- HRP Conjugate at -20°C or in a freezer.
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Feed
|
0.2
|
|
Fish/Shrimp/Meat
|
0.1
|
|
Egg/Egg Powder
|
0.1
|
|
Honey
|
0.1
|
|
Milk
|
0.1
|
|
Serum
|
0.1
|
|
Urine
|
0.1
|
Send Inquiry to this Member
Related Products of this Company
This member assumes full responsibility for the content of this listing. DIYTrade accepts no responsibility whatsoever in respect of such content.
To report fraudulent or illegal content, please
click here.
China Suppliers Quick Searching:
,
