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Synonyms

Heparan Sulfate

Intended use

This immunoassay kit allows for thein vitro quantitative determination ofgeneral Heparan Sulfate concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with anantibody specific to Heparan sulfate. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific forHeparan sulfateand Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heparan sulfate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparan sulfatein the samples is then determined by comparing the O.D. of the samples to the standard curve.