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Acridine ester is one of the most sensitive chemiluminescence reagents. Conventional acridine ester has 6 different groups, acridine ester NSP-SA-NHS, CAS number is 199293-83-9, product color is yellow It is in the form of powder. When it is used, it is configured to dissolve in contact with alkaline hydrogen peroxide, without the catalysis of enzymes, it can emit light by itself, because the contact between hydrogen peroxide and acridine ester changes the two components, which will produce luminescence. , collecting this photon can be used to label the detection compound.
Advantages of acridinium ester NSP-SA-NHS
1. Stable performance, high sensitivity, and purity of more than 98%;
2. The acridine ester with NHS active ester does not need a condensing agent, and the operation is simpler when marking;
3. Widely used, can be used for protein labeling, peptide labeling, etc.
Precautions for the use of acridinium ester NSP-SA-NHS
1. When packaging, use a dark bottle with good airtightness and opaqueness, because acridine ester must be stored at low temperature and must be tightly sealed, otherwise it will absorb moisture and deteriorate;
2. The active site of acridine ester NSP-SA-NHS itself is unstable in alkaline and oxidative environments, so it should be prepared and stored in a corresponding non-strong alkaline and non-oxidative system according to the stability of the labeled substance.
3. The composition of acridine ester will be affected under light, causing some substances to decompose. In order to avoid the subsequent luminous effect being affected, both the experimental process and the product storage should be strictly protected from light;
4. The entire luminescence process of acridine ester NSP-SA-NHS is very short (less than 2 seconds). Please prepare the detection instrument in advance when triggering the luminescence conditions.
Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of acridine esters. It has established a professional R&D team with reliable product quality. There are six different styles of acridine esters to choose from. If you need it, welcome to consult and order.