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Bio Excellence international Tech Co.,Ltd has been acting as sole agent for the sale of flexcell products for more than ten years,Bio Excellence can provide free technical guidance for flexcell tension, compression, fluid shear products, user site installation as well as training, until the user can use them without limiting number of training times.If you have any questions, please do not hesitate to contact us.If you have any questions, please do not hesitate to contact us.

 

Flexcell® FX-6000 Tension Systems

A computer-regulated bioreactor that uses vacuum pressure and positive air pressure to apply cyclic or static strain to cells cultured on flexible-bottomed culture plates.

• Applies a defined, controlled, static or cyclic deformation to cells growing in 3D or monolayer
• Allows users to simulate in vivo tissue strains and frequencies in vitro
• State-of-the-art digital valve automatically regulates vacuum pressure and positive air pressure for specified strain regimen
• Mimics in vivo conditions in cells from muscle, lung, heart, vascular vessels, skin, tendon, ligament, cartilage, and bone.
• FlexSoft® FX-6000 software allows for multiple frequency, amplitude and waveform changes to be programmed in one regimen
• Compatible with Windows 10
• Available waveforms: static, sinusoidal, heart stimulation, triangular, square and custom

Highlights

Apply equibiaxial or uniaxial tension to cells in 2D and 3D culture.

Simulate in vivo tissue strains and frequencies in an in vitro setting.

Contains state-of-the-art digital valves to automatically regulate and maintain pressure for a specified strain regimen.

Multiple frequency, amplitude and waveform changes can be programmed in one regimen.

Drives up to four independent FlexLink® remote compression and/or tension controllers.

Gradient strain or unconstrained distention achieved by removing loading stations

Drives up to 4 Flexlink® Controllers with 1 FX-6000T Tension System

Works with BioFlex® Culture Plate, UniFlex® Culture Plate,24-well HT BioFlex® Culture Plate, Tissue Train Circular Foam Culture Plate,Linear Tissue Train culture plates,Trapezoidal Tissue Train Culture Plate

 

Culture Plate for tension system	Max Achievable Strain	Strain Applications
(BioFLEX®CULTURE PLATES)	21.80%	with cylindrical loading posts to apply equibiaxial strain to cells in monolayer (2D)
UniFlexCulture Plates	12.20%	with Arctangle® loading posts to apply uniaxial strain to cells in monolayer (2D)
24 well HT BIOFLEX®CULTURE PLATES	15.00%	with 24 wells loading posts to apply equibiaxial strain to cells in monolayer (2D)
Tissue Train Circular Foam Culture Plate	1.6%-20.80%	with cylindrical loading posts to apply equibiaxial strain to 3D constructs
Linear Tissue Train culture plates	1.6%-20.80%	with Arctangle® loading posts to apply uniaxial strain to 3D constructs
Trapezoidal Tissue Train Culture Plate	1.6%-20.80%	with Arctangle® loading posts to apply uniaxial strain to 3D constructs
No Loading Stations	33%

 

 

 

Flexcell® Tissue Train® Systems

 

The Tissue Train® culture system is a stand-alone 3D culture system that allows investigators to create 3D geometries for cell culture in a matrix gel or allow the cells to build a self-assembled matrix that connects to the anchors in a Tissue Train® culture plate. Flexcell currently has molds and/or plates for creating three different shaped hydrogels: linear, trapezoidal, and circular.

The FX-5000™ Tension System provides the investigator with a tool to apply regulated uniaxial or equibiaxial strain to the growing bioartificial tissues. A user can define a frequency, elongation and duration of strain in a regimen that simulates the strain environment of the native tissue in the body.

 

ScanFlex with XyFlex

A measure of cellular remodeling of the extracellular matrix is gel compaction over time. ScanFlex is an automated image collection system that allows users to periodically scan items placed on a scanner bed. The ScanFlex software controls a digital scanner and allows users to program the number of times and the time intervals when digital scans are taken. When used in conjunction with Tissue Train® culture plates, ScanFlex can be used to determine the change in area of a bioartificial tissue (BAT). Furthermore, the area of a BAT can be measured using the XyFlex image analysis software. XyFlex software allows the user to automatically measure the BAT area in a large sequence of images.

 

Tissue Train® System includes:

· Host computer with monitor

· FlexSoft FX-5000™ software

· FX5K Tension FlexLink®

· Accessory package:

 BioFlex® baseplate and four gaskets

 Tissue Train® Trough Loaders

 Arctangle® Loading Stations

 Four Tissue Train® culture plates

 Drying filter, water trap, vacuum tubing, and grease/lubricant

 

Flexcell® FX-5000™ Compression Systems

 The FX-5000™ Compression System is a patented, computerized, pressure-operated instrument that applies a defined controlled, static or variable duration cyclic compression, to cells growing in vitro. This system utilizes regulated air pressure to deflect flexible-bottomed BioPress culture plates compressing a tissue sample or 3D cell culture between a piston and a stationary platen. This system can apply up to 14 pounds of applied force.

 

Highlights

Apply cyclic or static compression to cells in 3D culture.

Simulate in vivo tissue forces and frequencies in an in vitro setting.

Contains state-of-the-art digital valves to automatically regulate and maintain pressure for a specified compression regimen.

Works with BioPress culture plates.

Multiple frequency, amplitude and waveform changes can be programmed in one regimen.

Drives up to four independent FlexLink® remote compression and/or tension controllers.

 

FX-5000™ Compression System includes:

· Host computer with monitor

· FlexSoft FX-5000™ software

· FX5K Compression FlexLink®

· Compression accessory package:

   . BioPress baseplate and four gaskets

.Compression clamping system

.Four BioPress culture plates

.Tubing and quick disconnects

BioPress™ Compression Plates

Works with a Flexcell® Compression System to simulate biological compression conditions using pressure to deform tissue samples or cells cultured in a matrix.

• Uses positive pressure to compress tissue samples or cell-seeded constructs between a piston and stationary platen in the BioPress™ culture plate yielding up to 14 lb. of applied force
• Platens are reusable and autoclavable. They are sold separately and as part of the FX-5000C™ Compression System
• Biopress™ culture plates are available individually or in case quantity of 40 plates

Flexcell® Fluid Shear Systems

The Streamer® is a fluid shear stress device allowing users to regulate fluid shear stress to cells in culture with laminar, pulsatile, or oscillating flow. Flexcell’s® Streamer® System uses a computer-controlled peristaltic pump. Together with the Osci-Flow® Flow Controller, researchers can regulate the frequency of oscillation or pulsatile patterns based on shear stress level applied from 0-35 dynes/cm2. Flexcell's® Fluid Shear System is designed to allow users to observe biochemical changes, cell migration and signaling under fluid shear load in vitro.

 

Streamer

 

 

 

A system solution that allows users to apply fluid shear stress to cells in culture with laminar, pulsatile or oscillating flow.

• Uses a computer-controlled peristaltic pump to regulate shear stress from 0-35 dynes/cm2
• Works with the Osci-Flow® Flow Controller to regulate oscillating and pulsatile fluid flow
• Culture cells on special matrix-coated Teflon rimmed 25 mm × 75 mm × 1.0 mm Culture Slips®
• Analyze effects of fluid flow on cell alignment, mRNA and protein expression, and signaling pathways
• Remove with quick disconnect fittings for easy cleaning after use
• Streamer device is autoclavable
• Run up to six slides in parallel at one time

 

Osci-Flow® Flow Controller

A flow control device that gives the user the ability to regulate the frequency of oscillation or pulsatile patterns as well as shear stress levels applied to cells through StreamSoft® and NI-DAQ software.

• Provides software regulated control of oscillatory and pulsatile fluid flow
• Works as a system solution with the Streamer® and FlexFlow® shear stress devices
• Minimizes flow response lag by eliminating inertial effects of decelerating and accelerating pumps and motors
• Instantaneously reverses fluid flow
• Adapts to other perfusion systems
• Accommodates any MasterFlex L/S series, or comparable, tubing

FlexFlow®Shear®Stress Device

 

 

 

A parallel plate laminar shear stress device that allows the user to observe signaling responses to fluid flow and/or apply strain (up to 4%) to cells before, during, or after applying a shear stress.

• Test cell alignment response to fluid flow
• Culture your cells on matrix bonded surfaces using StageFlexer® Membranes or Culture Slips®.
• Regulate shear stress from 0-35 dynes/cm2 using a computer-controlled peristaltic pump
• Use with the Osci-Flow® Flow Controller to regulate the frequency of oscillation or pulsatile flow patterns

 

Culture Slips® Microscope Slides

Works with the Streamer® and FlexFlow® systems. Each slip is matrix treated to promote cell attachment.

• Culture Slips® are available in two sizes: 75 mm x 25 mm x 1.0 mm (for Streamer® or FlexFlow®), 76 mm x 25 mm x 1 mm (for FlexFlow® only)
• Culture Slips® are rimmed with a 1.0 mm wide Teflon® border to help limit cell culture growth to the portion of the slip exposed to fluid flow
• Covalently bound matrix surfaces: Untreated, Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR)
• Available in a pack of 6 or case of 36 culture slips

75mm x 25mm x 1.0mm for work with Streamer or FlexFlow 
Cat No     Product Name
CS-U      Culture Slips — Untreated 
CS-A      Culture Slips — Amino 
CS-C      Culture Slips — Collagen Type I 
CS-C(IV)  Culture Slips — Collagen Type IV 
CS-E      Culture Slips — Elastin 
CS-P      Culture Slips — ProNectin 
CS-L      Culture Slips — Laminin

75mm x 25mm x 0.2mm for work with  FlexFlow 

Cat No     Product Name
FFCS-U      Culture Slips — Untreated 
FFCS-A      Culture Slips — Amino 
FFCS-C      Culture Slips — Collagen Type I 
FFCS-C(IV)  Culture Slips — Collagen Type IV 
FFCS-E      Culture Slips — Elastin 
FFCS-P      Culture Slips — ProNectin 
FFCS-L      Culture Slips — Laminin 

 Flexcell Streamer Gold apparatus 

 

The Streamer® is a parallel-plate flow system that is used to apply fluid-induced shear stress to cells grown in a monolayer. The system includes a six-chamber laminar flow device designed to hold 75 x 25 x 1 mm Culture Slips®. The Streamer® system can be used to apply laminar, pulsatile*, or oscillating* flow to cells.  A computer-controlled peristaltic pump is used to regulate shear stress from 0-35 dynes/cm2.  This six place flow chamber can be used for various biological assays including, but not limited to, the assessment of RNA and protein expression by cells in response to fluid-induced shear stress and production of secreted molecules into the perfusate.

*When used in conjunction with the Osci-Flow® Flow Controller.

 

flexcell Osci-Flow flow controller

 

The Osci-Flow® is a software-controlled valve-operated device designed to provide oscillatory or start-stop flow patterns to cells cultured in the Streamer®, FlexFlow or other perfusion devices. The Osci-Flow® device is able to regulate flow both in an oscillatory (flow reversal) manner and in a pulsatile (square wave) mode, where the flow is stopped and then restarted after a designated period of time.  The StreamSoft software allows the user to regulate frequency of oscillation or pulsatile patterns, as well as shear stress levels applied to cells.

 

Flexcell FlexFlow combined cyclic stretch and fluid shear stress system

 

The FlexFlow is a parallel plate laminar flow device designed to apply fluid shear stress and/or cyclic strain to cells in culture while providing a means for viewing cell activity under a microscope in real time. The FlexFlow fits on the stage of a standard upright microscope. 

fluid shear Culture Slips® Microscope Slides

Works with the Streamer® and FlexFlow® systems. Each slip is matrix treated to promote cell attachment.

• Culture Slips® are available in two sizes: 75 mm x 25 mm x 1.0 mm (for Streamer® or FlexFlow®), 76 mm x 25 mm x 1 mm (for FlexFlow® only)
• Culture Slips® are rimmed with a 1.0 mm wide Teflon® border to help limit cell culture growth to the portion of the slip exposed to fluid flow
• Covalently bound matrix surfaces: Untreated, Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR)
• Available in a pack of 6 or case of 36 culture slips

75mm x 25mm x 1.0mm for work with Streamer or FlexFlow 
Cat No     Product Name
CS-U      Culture Slips — Untreated 
CS-A      Culture Slips — Amino 
CS-C      Culture Slips — Collagen Type I 
CS-C(IV)  Culture Slips — Collagen Type IV 
CS-E      Culture Slips — Elastin 
CS-P      Culture Slips — ProNectin 
CS-L      Culture Slips — Laminin

75mm x 25mm x 0.2mm for work with  FlexFlow 

Cat No     Product Name
FFCS-U      Culture Slips — Untreated 
FFCS-A      Culture Slips — Amino 
FFCS-C      Culture Slips — Collagen Type I 
FFCS-C(IV)  Culture Slips — Collagen Type IV 
FFCS-E      Culture Slips — Elastin 
FFCS-P      Culture Slips — ProNectin 
FFCS-L      Culture Slips — Laminin