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Brand: | MeltPro |
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Category: | Chemicals / Chemical Reagent |
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Live Chat: | Last Online:14 Dec, 2018 |
MeltPro® Mycobacterium tuberculosis Isoniazid-resistance Mutation Test Kit
Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is suitable for rapid, qualitative
detection of isoniazid-resistance mutations in Mycobacterium tuberculosis.
Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in Tm value between targets and the corresponding wild-type
probe sequences, the test detects the mutations in ahpC promoter region (positions -44~-30 and -15~3),
inhA codon 94, inhA promoter region (positions -17~-8), and katG codon 315, which reports
isoniazid-resistance information.
Detection region: mutations in ahpC promoter region (positions -44~-30 and -15~3), inhA codon 94,
inhA promoter region(positions -17~-8) and katG codon 315 (coverage rate: 50%-90% of all isoniazid-resistant mutants)
Principle of Probe-Based Melting Curve Analysis
Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest Tm value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as Tm deviation (△Tm ) compared to the wild-type hybrid.
Intended use: CFDA approved in China for clinical use, CE marked for IVD use in Europe.