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Tetracyclines Test Strip

Brief

This product is used for testing Tetracyclines residue in tissue samples (Fish, shrimp etc.). The whole test only need about 30 min, The sensitivity of Tetracyclines is 5ppb, suitable for all kinds of enterprises and Inspection agency.

Specifications:  40 tests/kit

Contents

Test cassette       

Manual      

Dropper   

Desiccant        

Sample preparation

Tissue (fish, shrimp etc.) sample should be in cold storage.

1 Remove fat from tissue, then mash it at high speed;

2 Weigh 5g sample into 50ml graduated centrifuge tube;

3 Add 3ml TCs A solution, shake violently for 5min;

4 Centrifuge at 4000 r/min at room temperature for 5min;

5 Move 2ml supernatant into another 5ml graduated centrifuge tube, add 150ul TCs B solution and 4ml Ethyl acetate, shake up-and- down evenly (do not shake violently) for 1 min, centrifuge at 4000 r/min at room temperature for 5min;

6 Use Micropipettes to absorb 3ml up-layer solution into another 5ml graduated centrifuge tube, blow to dry by air at 60°C.

7 Add 0.3ml TCS PBST buffer into the dry residue, use dropper beat and blow the liquid, and absorb the liquid to wash the inner well of the tube.

8 Use Micropipettes to absorb 120ul into gold micro well, ready to test.

Operation procedures

1 Return test card and sample into room temperature.

2 Take necessary strips on desk, use it in 1 hour.

3 Put the test card flatly, use dropper to drop 2 drops of sample (or use micropipette to take 80 uL) in the Sample collect region.

4 Wait for 3-5 min to read the result. (It’s valid in 10 minutes)

Test Result Interpretation

1 Negative: Red T line appears. It means there is no residue in sample or the residue is lower than detection limit.

2 Positive:Red T line is invisible. It means the residue is higher than detection limit.