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Chloramphenicol (CAP) ELISA test Kit

Brief

Chloramphenicol is a broad spectrum antibiotic which was widely used in veterinary medicine. Since 1994 the use of chloramphenicol is banned within the European Community.

Principle

The Baoankang™ Chloramphenicol (CAP) ELISA Assay Kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the tissue (chicken, pork), honey, milk, fish, shrimp and egg. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against Chloramphenicol After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Chloramphenicol in it. This value is compared to the standard curve and the content of the corresponding Chloramphenicol is subsequently obtained.

Specifications

Sensitivity: 0.05 ppb

Specifications: 96 wells/kit

Detection limit:

Intestine............................................................................................ 0.1 ppb

Honey............................................................................................... 0.1 ppb

Milk................................................................................................ 0.05 ppb

Urine, serum..................................................................................... 0.1 ppb

Tissue, liver................................................................................... 0.025 ppb

Feed.........................................................................0.15 ppb

Recovery rate

Tissue, liver.................................................................................... 80±10%

Honey, intestine.............................................................................. 70±10%

Milk, feed....................................................................................... 85±15%

Urine, serum......................................................................................70±10%

Cross-reaction rate:

Chloramphenicol................................................................................ 100%

Thiamphenicol................................................................................. < 0.1%

Florfeniol......................................................................................... < 0.1%

Storage: store at 2-8°C.not frozen

Shelf life: 12 months