| Ferment technique (60MT)
Ferment flow is belonged to typical Pichia pastoris semicontinuous fermentation, it contains 3 phases.
a)Seed phase
Generally, Seed phase has two kinds shaking bottle, 500L ferment tank seed, 6Ton ferment tank seed.
b)Thalli accumulating phase
In 60 tank, thalli use up all the carbon source and supplement carbon source, thalli reaches a some concentration.
c)Abduction phase
In the condiction of methanol FED-BATCH, the thalli is abducted to produce Phytate,and gradually accumulate.
Main equipment: fermentation tank,material storage tank, Air Compressor, boiler and auto-control equipment.
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Hopeland Chem-Tech Co., Ltd.
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